HCV E1 Antikörper

Produktnummer ABIN111760

Produktdetails anti-HCV E1 Antikörper

Target: HCV E1 (Hepatitis C Virus Envelope Protein E1 (HCV E1))

Reaktivität: Hepatitis C Virus (HCV)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HCV E1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Protein A Chromatography (Source: Ascites).

Immunogen: Recombinant HCV E1 glycoprotein (genotype 1b).

Klon: BDI198

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IFA. ELISA. Western blot. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2.0 mg/mL (OD280 nm, E0.1% = 1.3)

Buffer: 0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative without stabilizing proteins.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu HCV E1

Target: HCV E1 (Hepatitis C Virus Envelope Protein E1 (HCV E1))

Andere Bezeichnung: HCV Envelope Glycoprotein E1 (HCV E1 Produkte)

Substanzklasse: Viral Protein

Hintergrund: Envelope glycoproteins E1 and E2 are involved in virus attachment to the host cell as well as in virus endocytosis and fusion with host membrane. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.Synonyms: Heptitis C Virus E1, gp32, gp35