Selectin E/CD62e Antikörper (FITC)

Produktnummer ABIN111607

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Selectin E/CD62e in FACS. Er zeigt eine Reaktivität gegenüber Human und Schwein.

Kurzübersicht für Selectin E/CD62e Antikörper (FITC) (ABIN111607)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Selectin E/CD62e Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: 1-2B6

Produktdetails anti-Selectin E/CD62e Antikörper (FITC)

Spezifität: This antibody recognizes the CD62E and CD62P cell surface antigens. Although previously thought to recognise only Human CD62E, recent data shows that this antibody also recognizes Human CD62P, binding to a common epitope shared by these members of the selectin family.

Kreuzreaktivität (Details): Species reactivity (expected):Pig.
Species reactivity (tested):Human.
Also reacts to: CD62P

Aufreinigung: Affinity Chromatography on Protein A.

Immunogen: Human E-Selectin (ELAM-1). Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: use 10 μL of neat antibody to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Andere Bezeichnung: CD62E / E-Selectin

Hintergrund: CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

Gen-ID: 6401

NCBI Accession: NP_000441

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling