The antibody reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
NCAM1
Reaktivität: Human, Ratte, Huhn
FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Flow cytometry: use 20 l reagent / 100 l of whole blood or 10^6 cells in a suspension. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute only prior to immediate use
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody (undiluted) at 2-8 °C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.Synonyms: N-CAM-1, NCAM, NCAM-1, Neural cell adhesion molecule 1