EpCAM Antikörper

Produktnummer ABIN111180

Produktdetails anti-EpCAM Antikörper

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody recognises the 34 kDa cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: HT29 carcinoma cell line. Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.

Klon: HEA125

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 5 μL of neat antibody to label 10^6 cells or 100 μL cell suspension. Immunohistochemistry on Frozen Sections: 1/20. Immunohistochemistry on paraffin sections: 1/20. This product requires protein digestionpre-treatment of paraffin sections e. g.0.1 % pronase, 10 minutes. Recommended Positive Control: Human colon and appendix.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.3 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: CD326 / EPCAM / TACSTD1 (EPCAM Produkte)

Synonyme: DIAR5 antikoerper, EGP-2 antikoerper, EGP314 antikoerper, EGP40 antikoerper, ESA antikoerper, HNPCC8 antikoerper, KS1/4 antikoerper, KSA antikoerper, M4S1 antikoerper, MIC18 antikoerper, MK-1 antikoerper, TACSTD1 antikoerper, TROP1 antikoerper, ECS-1 antikoerper, ECS1 antikoerper, ESA1 antikoerper, M17S1 antikoerper, EGP antikoerper, Ep-CAM antikoerper, sb:cb6 antikoerper, tacstd antikoerper, wu:fj17g02 antikoerper, zgc:110304 antikoerper, zgc:77119 antikoerper, tacstd1 antikoerper, MGC80540 antikoerper, EPCAM antikoerper, egp antikoerper, ksa antikoerper, m4s1 antikoerper, mk-1 antikoerper, cd326 antikoerper, egp40 antikoerper, mic18 antikoerper, trop1 antikoerper, ep-cam antikoerper, hegp-2 antikoerper, co17-1a antikoerper, ga733-2 antikoerper, CD326 antikoerper, Egp314 antikoerper, EpCAM1 antikoerper, GA733-2 antikoerper, Ly74 antikoerper, Tacsd1 antikoerper, Tacstd1 antikoerper, gp40 antikoerper, epithelial cell adhesion molecule antikoerper, flotillin 2 antikoerper, epithelial cell adhesion molecule S homeolog antikoerper, EPCAM antikoerper, FLOT2 antikoerper, epcam antikoerper, epcam.S antikoerper, Epcam antikoerper

Hintergrund: This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1

Gen-ID: 4072

NCBI Accession: NP_002345

UniProt: P16422