KRT20 Antikörper (FITC)

Produktnummer ABIN111170

Der Maus Monoklonal Anti-KRT20-Antikörper wurde für IF, IHC (p), FACS und IHC (fro) validiert. Er ist geeignet, KRT20 in Proben von Human, Ratte und Schwein zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für KRT20 Antikörper (FITC) (ABIN111170)

Target: KRT20 (Keratin 20 (KRT20))

Reaktivität: Human, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT20 Antikörper ist konjugiert mit FITC

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: KS20-10

Produktdetails anti-KRT20 Antikörper (FITC)

Spezifität: This Monoclonal antibody represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide Recognized: Protein IT (keratin K20, Mr 46 000, also designated Cytokeratin 20). Tested Reactivities on Cultured Cell Lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.

Keine Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Species reactivity (tested):Human, Porcine (Pig) and Rat.

Aufreinigung: Affinity Chromatography

Immunogen: Electrophoretically purified Keratin K 20 from Human intestinal mucosa.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: FACS. Cytological Material. Immunohistochemistry on Frozen Sections. . Immunohistochemistry on Paraffin sections (Only after microwave treatment). Working Dilution: 1/10-1/20 in PBS, PH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT or over night at 2-8 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-KRT20 Antikörper (FITC) (ABIN111170)

Mușină, Zlei, Mentel, Scripcariu, Ștefan, Aniţei, Filip, Radu, Gavrilescu, Panuţa, Buna-Arvinte, Cordun, Predescu, Scripcariu, Huţanu: "Evaluation of circulating tumor cells in colorectal cancer using flow cytometry." in: The Journal of international medical research, Vol. 49, Issue 9, pp. 300060520980215, (2021) (PubMed).

Details zu KRT20

Target: KRT20 (Keratin 20 (KRT20))

Andere Bezeichnung: Cytokeratin 20

Hintergrund: Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT

Gen-ID: 54474

NCBI Accession: NP_061883

UniProt: P35900