Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

Vimentin Antikörper (pSer55)

Name: Vimentin Antikörper (pSer55)
SKU: ABIN1109485
Price: 940 EUR
Availability: InStock

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Vimentin in WB und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Western Blotting (WB) image for anti-Vimentin (VIM) (pSer55) antibody (ABIN1109485) (Vimentin Antikörper (pSer55))

Immunofluorescence (IF) image for anti-Vimentin (VIM) (pSer55) antibody (ABIN1109485) (Vimentin Antikörper (pSer55))

2 Abbildungen

Produktnummer ABIN1109485

Kurzübersicht für Vimentin Antikörper (pSer55) (ABIN1109485)

Target

Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)

Reaktivität

Human, Maus, Ratte

Wirt

Maus

Klonalität

Monoklonal

Konjugat

Dieser Vimentin Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunofluorescence (IF)

Klon

4A4

Discover our top product VIM Primärantikörper

Bindungsspezifität
34

32

17

16

12

10

9

7

5

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer55

Sequenz

SLYSS-phosphoS55-PGGAYC-KLH

Spezifität

This antibody reacts specifically with the phosphorylated MPV55 peptide but not the non-phosphorylated peptide. This antibody detects vimentin phosphorylated by cdc2 kinase and does not detect non-phosphorylated vimentin or phosphorylated vimentin by cAMP-dependent kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase II on Western blotting.

Kreuzreaktivität (Details)

Species reactivity (tested):Human, mouse, rat

Aufreinigung

Protein A agarose

Immunogen

Synthetic MPV55 phosphopeptide corresponding to mouse phosphorylated vimentin Ser55.

Isotyp

IgG2b
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Affe WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Huhn WB, IHC (p), ICC, IP Wirt: Maus Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Säugetier WB, FACS, IHC (p), ICC Wirt: Maus Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Maus Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, IF, IP Wirt: Kaninchen Monoclonal unconjugated

Applikationshinweise

Optimal working dilution should be determined by the investigator.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1.0 mg/mL

Buffer

PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Konservierungsmittel

Azide free

Handhabung

Avoid repeated freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Store (in aliquots) at -20 °C.
Target

Vimentin (VIM)

Andere Bezeichnung

Vimentin

Hintergrund

Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.Synonyms: VIM

Gen-ID

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Kaskade in der Apoptose