Ribonucleoprotein (RNP) Antikörper

Produktnummer ABIN1108879

Produktdetails

Target: Ribonucleoprotein (RNP)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Purified

Immunogen: Isolated nuclei. Splenocytes were fused with mouse myeloma NS-1 cells.

Klon: 58-15

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: Ribonucleoprotein (RNP)

Hintergrund: Anti-nuclear antibodies remain prevalent in a large group of autoimmune disorders. The accumulation of anti-nuclear antibodies is characteristic of lupus erythematosus, as well as various other auto-immune diseases such as Sjögren's syndrome, autoimmune hepatitis, dermatomyositis, rheumatoid arthritis, and scleroderma. Ribonucleoproteins (RNP) represent a 20-80nm electron dense nuclear structure, with highest labeling densities found in nuclear ribonucleoprotein (nRNP) particles. One of the main components of the nucleolus, RNPs are comprised of ribonucleic acid (RNA) and protein together, representing an RNA binding motif in an RNA binding protein. Aromatic amino acid residues occupying this RNP motif, create stacking interactions with RNA. Lysine residues expressed exclusively in the helical portion of RNA binding proteins stabilizes relationships with nucleic acids. The ability to quantitate and identify dividing cells facilitates immunopathologic studies on tissues from which prognostic information can be derived for disease states such as cancer.