Der Maus Monoklonal anti-POP7 Antikörper (Klon 1F11) (ABIN1108698) detektiert spezifisch POP7 in WB und EIA.
Dieser Antikörper reagiert spezifisch mit Proben aus Human.
Specificity has been tested in ELISA. In immunoblotting, under less stringent washing conditions, a weaker band of approximately 60 kDa is also detected (Figure 1, Right Panel). Additional tests for cross reactivity have not yet been performed.
Kreuzreaktivität (Details)
Species reactivity (tested):Human.
Aufreinigung
Purified
Immunogen
Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.
POP7
Reaktivität: Human
WB
Wirt: Maus
Polyclonal
unconjugated
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Dissolve in 200 μL buffer for a 0.5 mg/mL antibody concentration in 1 % BSA
Konzentration
0.5 mg/mL (when dissolved at 0.5 mg/mL, the BSA concentration will be 1%)
Buffer
10 mM Ammonium Bicarbonate buffer, 2 mg BSA
Vorsichtsmaßnahmen
Be careful opening the vial since the antibody resides in a Vacuum.
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C or Add up to 0.05% Sodium Azide for long term storage.
Target
POP7
(Processing of Precursor 7, Ribonuclease P/MRP Subunit (POP7))
Andere Bezeichnung
POP7
Hintergrund
The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.Synonyms: RPP20, Ribonuclease P protein subunit p20, Ribonucleases P/MRP protein subunit POP7 homolog, hPOP7