Fibronectin Antikörper

Produktnummer ABIN1107236

Der Maus Monoklonal Anti-Fibronectin-Antikörper wurde für WB, EIA und IHC (fro) validiert. Er ist geeignet, Fibronectin in Proben von Human, Ratte, Huhn, Kaninchen und Meerschweinchen zu detektieren.

Kurzübersicht für Fibronectin Antikörper (ABIN1107236)

Target: Fibronectin

Reaktivität: Human, Ratte, Huhn, Kaninchen, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: DH1

Produktdetails anti-Fibronectin Antikörper

Spezifität: The antibody is specific to extradomain A (EDA) sequence of a Cellular Fibronectin and recognizes thus only the Cellular Fibronectin (cFN). Clone DHI has been shown to specifically block Chondrocyte condensation in Chicken embryos (ref. 5).

Kreuzreaktivität (Details): Species reactivity (tested):Human, Chicken, Rat, Rabbit and Guinea Pig.

Aufreinigung: Ig Fraction

Immunogen: Purified Fibronectin from A8387 fibrosarcoma cells (ref. 21). Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.09 % (w/v) Sodium Azide, 1.0 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu Fibronectin

Target: Fibronectin

Hintergrund: Fibronectins are diverse high molecular weight cell adhesion glycoproteins found in basement membranes, in the interstitial connective tissue matrix, and in soluble in plasma and other body fluids. In adult tissues most fibronectin is produced by hepatocytes (pFn) that differ from locally produced cellular fibronectins. A monoclonal antibody (AM32091PU-N Clone BF12) has been produced to fibronectin that reacts with both the cellular and plasma forms of the protein and recognizes the cell-binding region in fibronectin. The monoclonal antibody DH1 recognizes only cellular form of Fibronectin. DH1 is specific to the extra domain A (EDA) of the molecule. cFns are found in some basement membranes during development and only in distinct tissues in adult, for instance in smooth muscle and alveolar walls of airways. cFns are abundant in the plasma of carcinoma patients and in the stroma of various carcinomas.Synonyms: CIG, Cold-insoluble globulin, FN1

Gen-ID: 2335

NCBI Accession: NP_002017

UniProt: P02751