Complex II Subunit 70 KDa Fp (C-Term) Antikörper

Produktnummer ABIN1106817

Produktdetails

Target: Complex II Subunit 70 KDa Fp

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Spezifität: This antibody detects Complex II subunit 70 kDa Fp (C-term). No cross reactivity with other proteins.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human, Rat.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of Human SDHA .Remarks: Sequence is identical to the related Rat and Mouse sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.2 % Sodium Acetate or Neutral PBS. If 0.5 mL of PBS is added the antibody concentration will be 0.1 mg/mL.

Buffer: 50 % Glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody after reconstitution at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: Complex II Subunit 70 KDa Fp

Hintergrund: Complex II of the mitochondrial respiratory chain, also known as succinate dehydrogenase or succinate:ubiquinone oxidoreductase, consists of 4 nuclear-encoded polypeptides, these are the flavoprotein subunit (SDHA), the iron sulfur protein subunit (SDHB), and the integral membrane protein subunits SDHC and SDHD. SDHA is an acronym for succinate dehydrogenase complex subunit A. The succinate dehydrogenase (SDH) protein complex catalyzes the oxidation of succinate (succinate + ubiquinone => fumarate + ubiquinol). The SDHA subunit is connected to the SDHB subunit on the hydrophilic, catalytic end of the complex, and weighs 72.7 kDA. Mutations in the SDHA subunit have a distinct pathology from mutations in the SDHB/SDHC/SDHD subunits, it is the only subunit to never have shown tumor suppressor behaviour. Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits. This appears to be due to the expression of two similar SDHA genes (Types I and II) in the paraganglia system.Synonyms: Mitochondria Complex II (Succinat Dehydrogenase) 70 kD Fp subunit, SDH2, SDHA, SDHF, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gen-ID: 6389

NCBI Accession: NP_004159

UniProt: P31040