CNF1 (AA 704-730) Antikörper

Produktnummer ABIN1106727

Produktdetails

Target: CNF1

Bindungsspezifität: AA 704-730

Reaktivität: E. coli

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Functional Studies (Func), Dot Blot (DB), Enzyme Immunoassay (EIA)

Spezifität: The Monoclonal antibody clone NG8 recognizes an epitope between amino acids 704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.

Immunogen: Synthetic peptide, corresponding to amino acids 704/730 of Escherichia coli CNF1

Klon: NG8

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: 0.1 % BSA

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Antigendetails

Target: CNF1

Hintergrund: CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho, however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.Synonyms: Cytotoxic necrotizing factor 1

Molekulargewicht: 115 kDa

Gen-ID: 3990770

NCBI Accession: YP_543855

UniProt: Q1R2U0