CKB Antikörper (N-Term)

Produktnummer ABIN1106703

Dieses Anti-CKB-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CKB in WB, IF, IHC (fro) und IHC (p). Geeignet für Human, Maus, Ratte und Kaninchen.

Kurzübersicht für CKB Antikörper (N-Term) (ABIN1106703)

Target: CKB (Creatine Kinase, Brain (CKB))

Reaktivität: Human, Maus, Ratte, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CKB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CK-BYK-21E10

Produktdetails anti-CKB Antikörper

Bindungsspezifität: N-Term

Sequenz: SNSHNALKLR FPAEDEF

Spezifität: The monoclonal antibody CK-BYK/21E10 recognizes creatine kinase B-type, also known as CKB.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat, rabbit

Aufreinigung: Protein G

Immunogen: Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain-type

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Details zu CKB

Target: CKB (Creatine Kinase, Brain (CKB))

Andere Bezeichnung: CKBB

Hintergrund: Human CKB is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes. Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development.Synonyms: B-CK, CKB, Creatine kinase B chain, Creatine kinase B-type, Creatine kinase BB

Gen-ID: 1152

NCBI Accession: NP_001814

UniProt: P12277