CD43 Antikörper (PE)

Produktnummer ABIN1106374

Produktdetails anti-CD43 Antikörper (PE)

Target: CD43 (SPN) (Sialophorin (SPN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: This antibody reacts with peripheral blood monocytes, lymphocytes and granulocytes. This antibody (clone 1D4) selectively recognizes core 2-containing O-glycans on CD43, which have the GlcNAcb1 (6 branch attached to the C-6 position of GalNAc) and are formed by core 2b-1,6-N-acetylglucosaminyltransferase (C2GnT). This type of CD43 is preferentially expressed in the CD45RO+ memory subset of CD4+ cells.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Protein-A Sepharose

Immunogen: PHA-activated human T-cell line maintained with IL-2 containing media

Klon: 1D4

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, 0.09 % NaN3, 1 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: CD43 / Leukosialin (SPN Produkte)

Hintergrund: Human CD43 antigen is a heavily sialylated single chain membrane glycoprotein with both molecular weight and antigenicity depending on the nature and complexity of the attached O-linked polysaccharides. Human CD43 is an integral membrane protein with a 235 amino acids (a.a.) extracellular domain, a transmembrane domain of 23 a.a and a 123 a.a. intracytoplasmic domain. The extracellular domain of CD43 has been shown to react with many ligands such as ICAM-1(CD54), E-selectin, galectin-1, MHC-I, and human serum albumin (HSA). The intracytoplasmic domain, which is highly conserved between species, is constitutively phosphorylated on serine residues. The level of phosphorylation increases after cell activation. This cell surface glycoprotein is also called leukosialin, sialophron or GPL115. A soluble form of CD43 is also present in human serum. Thymocytes, CD4+ T lymphocytes and monocytes express more of a 115 kDa CD43 isoform, whereas a 130 kDa isoform is found mostly on activated CD4+ T cells, CD8+ resting and activated T cells, neutrophils, platelets, and B cells. CD43 plays a role in the regulation of lymphoid cell adhesion, cellular maturation, and activation although the nature of this participation remains controversial. For example, the structural characteristics of the CD43 extracellular domain indicate that this molecule has both adhesive and anti-adhesive properties. O-glycosylated chains of CD43 such as a sialyl-Lewis x (sLex) epitope, a ligand for both P- and E-selectin, potentiate binding to lectin-like molecules. However, the adhesion molecule function for CD43 is counteracted by the presence of negatively charged sialic acid residues on its extracellular domain. In addition to the strong structural evidence for the involvement of CD43 in cell interactions, experimental data suggest contradictive role for CD43 in regulating cell contacts.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gen-ID: 6693

NCBI Accession: NP_001025459

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity