Myc Tag Antikörper

Details zu Produkt Nr. ABIN1105584, Anbieter: Anmelden zum Anzeigen
Antigen
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • ARC
  • B430311C09Rik
  • MYC
  • NOP
  • Nop30
  • MYC proto-oncogene, bHLH transcription factor
  • nucleolar protein 3 (apoptosis repressor with CARD domain)
  • MYC
  • Nol3
Reaktivität
Tag
211
58
14
12
6
5
3
1
1
Wirt
Maus
166
64
20
17
6
5
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
28
24
15
9
5
5
4
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
239
118
94
68
50
39
36
36
23
7
6
5
5
4
4
4
3
3
3
2
1
1
1
1
1
1
1
Optionen
Hersteller
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Immunogen GST-6myc-Tag fusion protein
Sequenz EQKLISEEDL
Klon PL14
Isotyp IgG1
Spezifität This antibody can be used for epitope-tagging using the amino acid sequence EQKLISEEDL.
Reinigung Protein-A Sepharose
Andere Bezeichnung C-Myc Epitope Tag
Substanzklasse Tag
Hintergrund Epitope tagging is a widely accepted technique that fuses an epitope peptide to a protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follows, YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which correspond to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.Synonyms: c-myc tag, myc tag, myc-tag
Gen-ID 4609
NCBI Accession NP_002458
UniProt P01106
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer PBS containing 50 % glycerol, without preservatives
Konservierungsmittel Without preservative
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store (in aliquots) at -20 °C.
Allgemeine Veröffentlichungen Heald, McLoughlin, McKeon: "Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase." in: Cell, Vol. 74, Issue 3, pp. 463-74, 1993 (PubMed).

Field, Nikawa, Broek, MacDonald, Rodgers, Wilson, Lerner, Wigler: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

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