ARHGEF2 Antikörper

Produktnummer ABIN1105439

Der Maus Monoklonal anti-ARHGEF2 Antikörper wird verwendet zum Nachweis von ARHGEF2 in Proben von Human, Maus und Hund. Er wurde validiert für WB, IF, IP und IHC (fro).

Kurzübersicht für ARHGEF2 Antikörper (ABIN1105439)

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Reaktivität: Human, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ARHGEF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: B4-7

Produktdetails anti-ARHGEF2 Antikörper

Spezifität: The monoclonal antibody B4/7 recognizes guanine nucleotide exchange factor H1 (GEF-H1).

Kreuzreaktivität (Details): Species reactivity (tested):Human, canine, mouse

Aufreinigung: Protein G

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Details zu ARHGEF2

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Andere Bezeichnung: ARHGEF2

Hintergrund: GEFH1 is an ~110 kDa protein belonging to the Dbl family of proto-oncogenes. GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of epithelial tight junctions and the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments. Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin. GEF-H1 is part of the signaling pathway connecting epithelial polarity with the cell cycle, and as such involved in oncogenesis.Synonyms: GEF-H1, Guanine nucleotide exchange factor H1, KIAA0651, LFP40, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40, Rho guanine nucleotide exchange factor 2

Gen-ID: 9181

NCBI Accession: NP_001155855

UniProt: Q92974

Pathways: Negative Regulation of intrinsic apoptotic Signaling