Actin Antikörper (C-Term, pan)

Produktnummer ABIN1105235

Der Kaninchen Polyklonal Anti-Actin-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, Actin in Proben von Human und Ratte zu detektieren.

Kurzübersicht für Actin Antikörper (C-Term, pan) (ABIN1105235)

Target: Actin

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Actin Antikörper

Bindungsspezifität: AA 367-377, C-Term, pan

Sequenz: Sequence is identical to the related rat sequence.

Kreuzreaktivität (Details): Species reactivity (tested):Human, rat

Aufreinigung: Immunogen affinity purified

Immunogen: Synthetic peptide corresponding to a sequence at the C-terminal of human Actin (367-377aa)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu Actin

Target: Actin

Hintergrund: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin,two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins,this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.