PLP1 Antikörper (C-Term)
Kurzübersicht für PLP1 Antikörper (C-Term) (ABIN1103681)
Target
Alle PLP1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- C-Term
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Marke
- IHC-plus™
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Spezifität
- Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.
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Aufreinigung
- Purified
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Immunogen
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Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.
Type of Immunogen: Synthetic peptide -
Isotyp
- IgG2a
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Applikationshinweise
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Approved: ICC, IHC, IHC-Fr, IHC-P (10 μg/mL), WB
Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: Can aggregate causing HMW bands to sometimes appear in certain preparations. Immunocytochemistry: 4 % PFA fixed cells for 5-10 minutes at RT. Use 0.1 % triton in block only to allow antibody access to C-terminal epitope. Immunohistochemistry (Frozen): Fresh frozen sections or 4 % PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2 % triton X-100 in the blocking step for permeabilization. Immunohistochemistry (Paraffin): Works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. Positive control: Brain. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- PBS buffer, pH 7.2, 0.09 % sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- avoid freeze thaw cycles. Store undiluted.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
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- PLP1 (Proteolipid Protein 1 (PLP1))
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Andere Bezeichnung
- PLP1 / Myelin PLP
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Hintergrund
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Name/Gene ID: PLP1
Synonyms: PLP1, HLD1, PMD, Myelin proteolipid protein, Proteolipid protein 1, SPG2, Lipophilin, MMPL, PLP, PLP/DM20 -
Gen-ID
- 5354
Target
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