PLP1 Antikörper (C-Term)

Produktnummer ABIN1103681

Der Maus Monoklonal Anti-PLP1 Antikörper wurde für WB, IHC, IHC (p), IHC (fro) und ICC validiert. Er ist geeignet, PLP1 in Proben von Human und Säugetier zu detektieren.

Kurzübersicht für PLP1 Antikörper (C-Term) (ABIN1103681)

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Reaktivität: Human, Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Produktdetails anti-PLP1 Antikörper

Bindungsspezifität: C-Term

Spezifität: Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.

Aufreinigung: Purified

Immunogen: Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.

Type of Immunogen: Synthetic peptide

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: Can aggregate causing HMW bands to sometimes appear in certain preparations. Immunocytochemistry: 4 % PFA fixed cells for 5-10 minutes at RT. Use 0.1 % triton in block only to allow antibody access to C-terminal epitope. Immunohistochemistry (Frozen): Fresh frozen sections or 4 % PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2 % triton X-100 in the blocking step for permeabilization. Immunohistochemistry (Paraffin): Works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. Positive control: Brain.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS buffer,  pH 7.2, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Details zu PLP1

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Andere Bezeichnung: PLP1 / Myelin PLP

Hintergrund: Name/Gene ID: PLP1

Synonyms: PLP1, HLD1, PMD, Myelin proteolipid protein, Proteolipid protein 1, SPG2, Lipophilin, MMPL, PLP, PLP/DM20

Gen-ID: 5354