LINE-1 Antikörper

Produktnummer ABIN1102848

Der Huhn Polyklonal anti-LINE-1 Antikörper (ABIN1102848) detektiert spezifisch LINE-1 in ELISA und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für LINE-1 Antikörper (ABIN1102848)

Target: LINE-1

Reaktivität: Human

Wirt: Huhn

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Produktdetails

Spezifität: Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100 % homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88 % homology with the immunizing sequences.

Aufreinigung: Protein A purified

Sterilität: Sterile filtered

Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.

Type of Immunogen: Synthetic peptide

Isotyp: IgY

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (1:2000 - 1:10000)

Usage: This IgY fraction antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149 kD in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Antigendetails

Target: LINE-1