Malic Dehydrogenase (MDH) Antikörper

Produktnummer ABIN1099697

Der Kaninchen Polyklonal Anti--Antikörper wurde für IP validiert. Er ist geeignet, in Proben von Schwein zu detektieren.

Kurzübersicht für Malic Dehydrogenase (MDH) Antikörper (ABIN1099697)

Target: Malic Dehydrogenase (MDH)

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunoprecipitation (IP)

Produktdetails

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Physicochemical characteristics: PAb concentration 1 mg/0.5ml. No foreign proteins added.

Aufreinigung: Antisera
Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.

Immunogen: Malic dehydrogenase isolated and purified from porcine heart. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/10,000 and 1/100,000.

Kommentare:

Physical form: Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Phosphate buffered saline (PBS, pH 7.2). No foreign protein added.

Konservierungsmittel: Without preservative

Handhabung: The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Lagerung: 4 °C

Antigendetails

Target: Malic Dehydrogenase (MDH)

Hintergrund: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.