Der Maus Monoklonal anti-Zona Radiata Protein Antikörper wird verwendet zum Nachweis von Zona Radiata Protein in Proben von Atlantic Salmon. Er wurde validiert für ELISA und WB.
Kurzübersicht für Zona Radiata Protein Antikörper (ABIN108740)
Target
Zona Radiata Protein
Reaktivität
Atlantic Salmon
Wirt
Maus
Klonalität
Monoklonal
Konjugat
Unkonjugiert
Applikation
ELISA, Western Blotting (WB)
Klon
MN-2B4
Spezifität
The monoclonal antibody MN-2B4 binds with high affinity to zona radiata proteins (Zrp) in plasma samples from Atlantic salmon (Salmo salar), reacting primarily with the b monomer. The antibody also cross-reacts with Zrp from rainbow trout (Oncorhynchus mykiss) and Arctic charr (Salvelinus alpinus). The degree of cross-reactivity differs between species and with the methods employed.
Immunogen
Immunogen source: Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from Atlantic salmon (Salmo salar).
The monoclonal antibody MN-2B4 works well in both ELISA and western blot. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating. Normal dilution range: ELISA: 1:100 - 1: 5000. Western blot: 1:100 - 1: 1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Lagerung
4 °C
Target
Zona Radiata Protein
Hintergrund
This package contains one vial of 100 µl antibody solution. The antibody is dissolved in 0.1 M Na-phosphate buffer, 0.15 M NaCl, 1 % BSA, 12.3 mM Na-azide, pH 7.4.