PABP Antikörper

Produktnummer ABIN108538

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PABP in WB, ELISA, IF, IP, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human, Kaninchen, Huhn, Xenopus laevis und Hamster und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für PABP Antikörper (ABIN108538)

Target: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Reaktivität: Human, Kaninchen, Huhn, Xenopus laevis, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PABP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 10E10

Produktdetails anti-PABP Antikörper

Aufreinigung: Protein G

Immunogen: Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal antibody dilution should be determined by titration, however try:WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa)ICC/IF: Use at an assay dependent dilution. PubMed: 17977970Flow Cyt: Use 1 μg for 106 cells

Kommentare:

Myeloma, fusion partners: SP2/0

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-PABP Antikörper (ABIN108538)

Gorgoni, Richardson, Burgess, Anderson, Wilkie, Gautier, Martins, Brook, Sheets, Gray: "Poly(A)-binding proteins are functionally distinct and have essential roles during vertebrate development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 19, pp. 7844-9, (2011) (PubMed).

Unsworth, Raguz, Edwards, Higgins, Yagüe: "mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 9, pp. 3370-80, (2010) (PubMed).

Görlach, Burd, Dreyfuss: "The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity." in: Experimental cell research, Vol. 211, Issue 2, pp. 400-7, (1994) (PubMed).

Details zu PABP

Target: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Andere Bezeichnung: PABP

Hintergrund: Synonyms:
PAB 1 antibody,PAB1 antibody,PABP 1 antibody,PABP1 antibody,PABPC 1 antibody,PABPC1antibody,PABPC2 antibody,PABPL1 antibody,Poly A binding protein 1 antibody,Poly A binding protein cytoplasmic1 antibody,Polyadenylate binding protein 1 antibody

Gen-ID: 117282

OMIM: 604679

UniProt: P22626

Pathways: SARS-CoV-2 Protein Interaktom