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Uricase (ABC3735) Antikörper (Biotin)

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch in ELISA, IP und WB. Er zeigt eine Reaktivität gegenüber Bacillus.
Rockland
Produktnummer ABIN108081
Hersteller Produkt- Nr.: 200-106-092-0100

Kurzübersicht für Uricase (ABC3735) Antikörper (Biotin) (ABIN108081)

Target

Uricase (ABC3735)

Reaktivität

Bacillus

Wirt

  • 3
Ziege

Klonalität

  • 3
Polyklonal

Konjugat

  • 2
  • 1
Biotin

Applikation

ELISA, Immunoprecipitation (IP), Western Blotting (WB)
  • Hersteller Produkt- Nr.

    200-106-092-0100

    Hersteller

    Rockland

    Verwendungszweck

    Anti-Uricase Antibody Biotin Conjugated

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].

    Produktmerkmale

    Synonyms: goat anti-uricase antibody, biotin conjugated goat anti-uricase antibody, Urate oxidase antibody, Factor-independent urate hydroxylase

    Aufreinigung

    Anti-Uricase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogen

    Immunogen: Uricase [Bacillus species]

    Immunogen Type: Native Protein

    Isotyp

    IgG
  • Applikationshinweise

    Application Note: Anti-Uricase Antibody has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 μg of Uricase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:53,000 of the reconstitution concentration is suggested for Anti-Uricase Antibody.

    Western Blot Dilution: 1:500 - 1:1,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:1,000 - 1:2,500

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    10.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Haltbarkeit

    12 months
  • Target

    Uricase (ABC3735)

    Andere Bezeichnung

    ABC3735

    Substanzklasse

    Dinucleotide

    Hintergrund

    Background: Uricase is a peroxisomal enzyme that catalyzes the conversion of urea to allantoin. Uricase performs this action by cleaving the purine ring of uric acid rendering it much more soluble within the body for excretion. Uricase within the Bacillus species is of high importance due to its high activity and thermostability over a wide range of pH 's. Anti-Uricase Antibody is ideal for investigators in Cell Biology and Microbiology research.

    Gen-ID

    3202700

    NCBI Accession

    YP_177228

    UniProt

    Q5WBJ3
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