Urease Antikörper

Produktnummer ABIN108075

Produktdetails anti-Urease Antikörper

Target: Urease (URE)

Reaktivität: Schwertbohne

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Urease Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.

Reinheit: Anti-UREASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Urease [Jack Bean]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-UREASE Antibody has been assayed against 1.0 µg of Urease [Jack Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:60.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Urease

Target: Urease (URE)

Andere Bezeichnung: Urease (URE Produkte)

Synonyme: F12B7.10 antikoerper, F12B7_10 antikoerper, urease antikoerper, urease antikoerper, URE antikoerper, CNH01900 antikoerper

Hintergrund: Synonyms: Urea Amidohydrolase antibody

UniProt: P07374