Endothelin 1 Antikörper (AA 54-202)

Produktnummer ABIN1077983

Produktdetails anti-Endothelin 1 Antikörper

Target: Endothelin 1 (EDN1)

Bindungsspezifität: AA 54-202

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Endothelin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Verwendungszweck: Polyclonal Antibody to Endothelin 1 (EDN1)

Spezifität: The antibody is a rabbit polyclonal antibody raised against EDN1. It has been selected for its ability to recognize EDN1 in immunohistochemical staining and western blotting.

Kreuzreaktivität: Maus

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA482Ra01Recombinant Endothelin 1 (EDN1)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Endothelin 1

Target: Endothelin 1 (EDN1)

Andere Bezeichnung: EDN1 (EDN1 Produkte)

Hintergrund: ET1, PPET1, Preproendothelin-1, Big endothelin-1

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size