Protein G Antikörper

Produktnummer ABIN106214

Produktdetails anti-Protein G Antikörper

Target: Protein G

Reaktivität: Streptococcus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Protein G Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): Cross reactivity occurs against Protein A.

Reinheit: Anti-PROTEIN G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Protein G.

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Protein G [Streptococcus species]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-PROTEIN G is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20.000 - 1:100.000
Western Blot Dilution: 1:2.000 - 1:10.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Protein G

Target: Protein G

Abstract: Protein G Produkte