Phosphotyrosine Antikörper

Produktnummer ABIN105624

Hersteller Produkt- Nr.: 500-301-262

Produktdetails anti-Phosphotyrosine Antikörper

Target: Phosphotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 500-301-262

Hersteller: Rockland

Verwendungszweck: Phosphotyrosine Antibody

Kreuzreaktivität (Details): Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.

Produktmerkmale: Synonyms: mouse anti-Phosphotyrosine Antibody, mouse anti-pY antibody, PhosphoTyrosine reactive antibody, monoclonal phosphotyrosine, anti-phosphotyrosine produced in mouse

Aufreinigung: This product is clarified ascites produced in balb/c mice using clone 13F9.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This monoclonal antibody was produced after repeated immunizations of balb/c mice with phosphotyrosine conjugated KLH.

Immunogen Type: Other

Klon: 13F9

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Application Note: This monoclonal antibody has been tested in ELISA and Western Blot and reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine.  The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation.    Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Produkte

Substanzklasse: Amino Acid

Hintergrund: Background: Tyrosine (Tyr or Y) or 4-hydroxyphenylalanine is a hydrophilic amino acid. In plants and most microorganisms, tyrosine and α-ketoglutarate are produced via prephenate, when oxidatively decarboxylated with retention of the hydroxyl group to give p-hydroxyphenylpyruvate, which is transaminated using glutamate as the nitrogen source. Mammals synthesize tyrosine from the essential amino acid phenylalanine (phe), which is derived from food. The conversion of phe to tyr is catalyzed by the enzyme phenylalanine hydroxylase causing the addition of a hydroxyl group. In its phosphorylated form, tyrosine is called phosphotyrosine. Tyrosine phosphorylation is one of the key steps in signal transduction and regulation of enzymatic activity. Protein kinases can tag on the hydroxyl group end with a phosphate group to some tyrosine residues causing a phosphorylated form. Tyrosine residues may also be modified by the addition of a sulfate group, a process known as tyrosine sulfation.