Phosphothreonine Antikörper

Produktnummer ABIN105601

Hersteller Produkt- Nr.: 500-301-263

Produktdetails anti-Phosphothreonine Antikörper

Target: Phosphothreonine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphothreonine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 500-301-263

Hersteller: Rockland

Verwendungszweck: Phosphothreonine Antibody

Kreuzreaktivität (Details): Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.

Produktmerkmale: Synonyms: mouse anti-Phosphothreonine Antibody, mouse anti-pT antibody

Aufreinigung: This product is clarified ascites produced in balb/c mice using clone 18F6.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Phosphothreonine antibody was produced after repeated immunizations of balb/c mice with phosphothreonine conjugated KLH.

Immunogen Type: Other

Klon: 18F6

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Application Note: This monoclonal antibody has been tested in ELISA and Western Blot and reacts specifically with phosphothreonine and shows minimal reactivity by ELISA, and competitive ELISA, with phosphoserine or phosphotyrosine.  The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, as well as phosphothreonine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation.  Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases and is important for cell signaling in activation, proliferation and differentiation.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Produkte

Substanzklasse: Amino Acid

Hintergrund: Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.