PPY Antikörper

Produktnummer ABIN105318

Hersteller Produkt- Nr.: 214-a01-118

Dieses Anti-PPY-Antikörper ist ein Hund Polyklonal-Antikörper zur Detektion von PPY in . Geeignet für Schwein.

Kurzübersicht für PPY Antikörper (ABIN105318)

Target: PPY (Pancreatic Polypeptide (PPY))

Reaktivität: Schwein

Wirt: Hund

Klonalität: Polyklonal

Konjugat: Dieser PPY Antikörper ist unkonjugiert

Applikation: Bitte anfragen

Produktdetails anti-PPY Antikörper

Hersteller Produkt- Nr.: 214-a01-118

Hersteller: Rockland

Verwendungszweck: Pancreatic Islet Lysate Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum.

Produktmerkmale: Synonyms: IgG fraction of Anti-pancreatic islet lysate, Dog Anti-Pancreatic lysate (porcine pancreas), Anti-pancreatic lysate produced in dog, dog anti-Pancreatic Islet Lysate Antibody, canine anti-Pancreatic Islet Lysate Antibody

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optional[Flow Cytometry Dilution]: This product has been assayed against 1.0 μg of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and o-Phenylenediamine (OPD) as a substrate.  A working dilution of 1:50 to 1:100 is suggested for this product.  This product has been assayed against 1.5 μg of Pancreatic Islet Lysate [Porcine Pancreas] per lane by immunoblot (western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) code #604-1302 and Diaminobenzidine (DAB) as a substrate.  A working dilution of 1:50 to 1:200 is suggested for this product.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PPY

Target: PPY (Pancreatic Polypeptide (PPY))

Andere Bezeichnung: PPY

Hintergrund: Background: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. It may be used as a marker of the viability of xenotransplanted fetal pancreatic tissue in the immediate post-transplant period.

UniProt: P01300

Pathways: Feeding Behaviour