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VIPR1 Antikörper (Cytoplasmic Domain)

VIPR1 Reaktivität: Human IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049454
  • Target Alle VIPR1 Antikörper anzeigen
    VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))
    Bindungsspezifität
    • 15
    • 13
    • 9
    • 8
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reaktivität
    • 48
    • 26
    • 26
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 63
    • 2
    • 1
    Kaninchen
    Klonalität
    • 66
    Polyklonal
    Konjugat
    • 27
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser VIPR1 Antikörper ist unkonjugiert
    Applikation
    • 56
    • 25
    • 22
    • 22
    • 7
    • 7
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human VIP Receptor 1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GHRHR (56 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Monkey, Marmoset, Rabbit (94%) Hamster, Pig (81%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human VIP Receptor 1. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Monkey, Marmoset, Rabbit (94%), Hamster, Pig (81%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product VIPR1 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))
    Andere Bezeichnung
    VIPR1 (VIPR1 Produkte)
    Synonyme
    HVR1 antikoerper, II antikoerper, PACAP-R-2 antikoerper, PACAP-R2 antikoerper, RDC1 antikoerper, V1RG antikoerper, VAPC1 antikoerper, VIP-R-1 antikoerper, VIPR antikoerper, VIRG antikoerper, VPAC1 antikoerper, VPAC1R antikoerper, VPCAP1R antikoerper, CVIPR antikoerper, AV071699 antikoerper, VIP-R1 antikoerper, RATVASREC antikoerper, VASREC antikoerper, VIPR1 antikoerper, LOC100226500 antikoerper, vipr1 antikoerper, hvr1 antikoerper, rdc1 antikoerper, v1rg antikoerper, vipr antikoerper, virg antikoerper, vapc1 antikoerper, vpac1 antikoerper, vpcap1 antikoerper, vpac1-r antikoerper, VIP-R antikoerper, vasoactive intestinal peptide receptor 1 antikoerper, vasoactive intestinal peptide receptor 1b antikoerper, vasoactive intestinal peptide receptor 1 S homeolog antikoerper, vasoactive intestinal polypeptide receptor antikoerper, VIPR1 antikoerper, Vipr1 antikoerper, vipr1b antikoerper, vipr1 antikoerper, vipr1.S antikoerper, LOC100303683 antikoerper
    Hintergrund
    Name/Gene ID: VIPR1
    Subfamily: Vasoactive intestinal polypeptide
    Family: GPCR

    Synonyms: VIPR1, HVR1, PACAP type II receptor, PACAP-R-2, Pvr2, RDC1, Vip receptor subtype 1, Vpac1 receptor, V1RG, VIP receptor 1, VIPR, VIRG, VPCAP1R, PACAP-R2, VAPC1, VIP and PACAP receptor 1, VPAC1R, Pacap receptor, type ii, Type II PACAP receptor, VIP receptor, type I, VIP-R-1, VIP1 receptor, VPAC1
    Gen-ID
    7433
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