Thromboxane A2 Receptor (TBXA2R) (Cytoplasmic Domain) Antikörper

Details zu Produkt Nr. ABIN1049407, Anbieter: Anmelden zum Anzeigen
Antigen
  • tbxa2r
  • MGC80805
  • TBXA2R
  • BDPLT13
  • TXA2-R
  • TP
  • thromboxane A2 receptor L homeolog
  • thromboxane A2 receptor
  • tbxa2r.L
  • TBXA2R
  • tbxa2r
  • Tbxa2r
Epitop
Cytoplasmic Domain
6
3
3
2
2
2
2
1
1
1
1
1
Reaktivität
Human
40
7
6
3
2
1
Wirt
Kaninchen
38
3
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
1
1
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
22
14
14
9
4
2
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke IHC-plus™
Immunogen Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human TBXA2R. Percent identity with other species by BLAST analysis: Human, Gorilla, Panda (100%), Gibbon, Monkey, Marmoset, Bovine (94%), Bat, Elephant (88%), Dog (81%).

Type of Immunogen: Synthetic peptide
Spezifität Human TBXA2R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Panda (100%) Gibbon, Monkey, Marmoset, Bovine (94%) Bat, Elephant (88%) Dog (81%).
Reinigung Immunoaffinity purified
Antigen
Andere Bezeichnung TBXA2R/Thromboxane A2 Receptor (TBXA2R Antibody Abstract)
Hintergrund Name/Gene ID: TBXA2R
Subfamily: Prostanoid
Family: GPCR

Synonyms: TBXA2R, BDPLT13, Prostanoid TP receptor, Txa2 receptor, Thromboxane A2 receptor, TXA2-R
Gen-ID 6915
Applikations-hinweise Approved: IHC, IHC-P (20 μg/mL)
Kommentare

Target Species of Antibody: Human

Testdurchführung

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Thromboxane A2 Receptor (TBXA2R) (Cytoplasmic Domain) antibody (ABIN1049407) Anti-TBXA2R antibody ABIN1049407 IHC staining of human uterus. Immunohistochemistry ...
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