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QRFPR Antikörper (N-Term)

This anti-QRFPR antibody (ABIN1049284) is a Rabbit Polyclonal antibody detecting QRFPR in IHC (p), IHC. Suitable for Human, Cow, Horse, Rabbit, Pig.
Produktnummer ABIN1049284

Kurzübersicht für QRFPR Antikörper (N-Term) (ABIN1049284)

Target

Alle QRFPR Antikörper anzeigen
QRFPR (Pyroglutamylated RFamide Peptide Receptor (QRFPR))

Reaktivität

  • 48
  • 17
  • 13
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Rind (Kuh), Pferd, Kaninchen, Schwein

Wirt

  • 55
Kaninchen

Klonalität

  • 55
Polyklonal

Konjugat

  • 24
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser QRFPR Antikörper ist unkonjugiert

Applikation

  • 38
  • 19
  • 13
  • 13
  • 8
  • 6
  • 4
  • 3
  • 2
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 15
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Marke

    IHC-plus™

    Spezifität

    Human QRFPR / GPR103. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

    Homologie

    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Bovine, Horse, Rabbit, Pig (100%) Monkey, Rat, Dog, Elephant (95%) Mouse, Panda (90%) Bat, Turkey, Zebra finch, Chicken, Platypus, Xenopus (85%) Opossum (80%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic 20 amino acid peptide from N-Terminus of human QRFPR / GPR103. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Bovine, Horse, Rabbit, Pig (100%), Monkey, Rat, Dog, Elephant (95%), Mouse, Panda (90%), Bat, Turkey, Zebra finch, Chicken, Platypus, Xenopus (85%), Opossum (80%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise

    Approved: IHC, IHC-P (8 μg/mL)

    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    PBS, less than 0.1 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    QRFPR (Pyroglutamylated RFamide Peptide Receptor (QRFPR))

    Andere Bezeichnung

    QRFPR / GPR103

    Hintergrund

    Name/Gene ID: QRFPR
    Subfamily: Orphan-U
    Family: GPCR

    Synonyms: QRFPR, AQ27, G protein-coupled receptor 103, QRFP receptor, SP9155, G-protein coupled receptor 103, GPR103

    Gen-ID

    84109
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