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Prostacyclin Receptor Antikörper (N-Term)

Der Kaninchen Polyklonal Anti-Prostacyclin Receptor-Antikörper wurde für IHC und IHC (p) validiert. Er ist geeignet, Prostacyclin Receptor in Proben von Human, Affe und Kaninchen zu detektieren.
Produktnummer ABIN1049252

Kurzübersicht für Prostacyclin Receptor Antikörper (N-Term) (ABIN1049252)

Target

Alle Prostacyclin Receptor (PTGIR) Antikörper anzeigen
Prostacyclin Receptor (PTGIR)

Reaktivität

  • 43
  • 11
  • 5
  • 1
  • 1
Human, Affe, Kaninchen

Wirt

  • 44
  • 4
Kaninchen

Klonalität

  • 44
  • 4
Polyklonal

Konjugat

  • 21
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Prostacyclin Receptor Antikörper ist unkonjugiert

Applikation

  • 24
  • 16
  • 14
  • 13
  • 13
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 15
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Marke

    IHC-plus™

    Spezifität

    Human Prostaglandin I2 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

    Homologie

    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Rabbit (100%) Panda, Dog, Bovine (93%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic 15 amino acid peptide from N-terminal extracellular domain of human Prostaglandin I2 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Rabbit (100%), Panda, Dog, Bovine (93%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise

    Approved: IHC, IHC-P (14 μg/mL)

    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    PBS, less than 0.1 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    Prostacyclin Receptor (PTGIR)

    Andere Bezeichnung

    PTGIR / IP Receptor

    Hintergrund

    Name/Gene ID: PTGIR
    Subfamily: Prostanoid
    Family: GPCR

    Synonyms: PTGIR, IP receptor, PGI receptor, PRIPR, Prostaglandin I2 receptor, IP, PGI2 receptor, Prostacyclin IP receptor, Prostacyclin receptor, Prostanoid IP receptor

    Gen-ID

    5739

    Pathways

    cAMP Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling
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