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PAK6 Antikörper (Linker Domain)

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch PAK6 in IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Pferd, Hund und Affe.
Produktnummer ABIN1049227
534,81 €
Zzgl. Versandkosten 20,00 € und MwSt
50 μg
Lieferung nach: Deutschland
Lieferung in 15 bis 19 Werktagen

Kurzübersicht für PAK6 Antikörper (Linker Domain) (ABIN1049227)

Target

Alle PAK6 Antikörper anzeigen
PAK6 (P21-Activated Kinase 6 (PAK6))

Reaktivität

  • 60
  • 40
  • 6
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Pferd, Hund, Affe

Wirt

  • 75
Kaninchen

Klonalität

  • 74
Polyklonal

Konjugat

  • 35
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser PAK6 Antikörper ist unkonjugiert

Applikation

  • 50
  • 26
  • 26
  • 26
  • 13
  • 13
  • 11
  • 6
  • 6
  • 4
  • 3
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 15
    • 15
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Linker Domain

    Spezifität

    Human PAK6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

    Homologie

    Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Horse (100%) Goat, Elephant, Panda, Rabbit (94%) Hamster, Bovine (89%) Mouse, Bat (83%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic 18 amino acid peptide from Linker domain of human PAK6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Horse (100%), Goat, Elephant, Panda, Rabbit (94%), Hamster, Bovine (89%), Mouse, Bat (83%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise

    Approved: IHC, IHC-P (3.5 μg/mL)

    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    PBS, less than 0.1 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    PAK6 (P21-Activated Kinase 6 (PAK6))

    Andere Bezeichnung

    PAK6

    Hintergrund

    Name/Gene ID: PAK6
    Subfamily: PAK (STE20)
    Family: Protein Kinase

    Synonyms: PAK6, PAK5, p21-activated kinase 6, p21-activated protein kinase 6, PAK-5, PAK-6, p21(CDKN1A)-activated kinase 6

    Gen-ID

    56924
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