OXGR1 Antikörper (Extracellular Domain)

Produktnummer ABIN1049192

Produktdetails anti-OXGR1 Antikörper

Target: OXGR1 (Oxoglutarate (Alpha-Ketoglutarate) Receptor 1 (OXGR1))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OXGR1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marke: IHC-plus™

Spezifität: Human OXGR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Gibbon, Dog, Bat, Elephant, Panda, Horse (94%) Mouse, Rat, Bovine (88%) Rabbit (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd extracellular domain of human OXGR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Gibbon, Dog, Bat, Elephant, Panda, Horse (94%), Mouse, Rat, Bovine (88%), Rabbit (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (22 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu OXGR1

Target: OXGR1 (Oxoglutarate (Alpha-Ketoglutarate) Receptor 1 (OXGR1))

Andere Bezeichnung: GPR80 / GPR99 / OXGR1 (OXGR1 Produkte)

Synonyme: GPR80 antikoerper, GPR99 antikoerper, P2RY15 antikoerper, P2Y15 antikoerper, Cysltr3 antikoerper, Gm299 antikoerper, Gpr80 antikoerper, Gpr99 antikoerper, oxoglutarate receptor 1 antikoerper, oxoglutarate (alpha-ketoglutarate) receptor 1 antikoerper, OXGR1 antikoerper, Oxgr1 antikoerper

Hintergrund: Name/Gene ID: OXGR1
Subfamily: Purinergic
Family: GPCR

Synonyms: OXGR1, 2-oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, G protein-coupled receptor 80, GPR80, G-protein coupled receptor 99, GPR99, p2Y purinoceptor 15, p2Y-like nucleotide receptor, p2RY15, Oxoglutarate receptor 1, p2Y-like GPCR, p2Y15, G protein-coupled receptor 99, G-protein coupled receptor 80

Gen-ID: 27199