OR2A4 Antikörper (Olfactory Receptor 2A4) (Extracellular Domain)

Details for Product anti-OR2A4 Antibody No. ABIN1049177, Anbieter: Anmelden zum Anzeigen
Antigen
  • OR2A10
  • olfactory receptor family 2 subfamily A member 4
  • OR2A4
Epitop
Extracellular Domain
11
10
7
3
3
2
2
2
2
2
2
1
1
Reaktivität
Human
61
1
1
1
Wirt
Kaninchen
61
Klonalität
Polyklonal
Konjugat
Dieser OR2A4 Antikörper ist unkonjugiert
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
29
19
17
13
8
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke IHC-plus™
Immunogen Synthetic 14 amino acid peptide from 2nd extracellular domain of human OR2A4. Percent identity with other species by BLAST analysis: Human (100%), Horse (93%), Marmoset, Panda, Dog, Opossum (86%).

Type of Immunogen: Synthetic peptide
Spezifität Human OR2A4. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except OR2T35 (71 % ), OR2T2 (71 %).
Homologie Percent identity with other species by BLAST analysis: Human (100%) Horse (93%) Marmoset, Panda, Dog, Opossum (86%).
Reinigung Immunoaffinity purified
Plasmids, Primers & others Plasmide, Primers & weitere OR2A4 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung OR2A4 (OR2A4 Antibody Abstract)
Hintergrund Name/Gene ID: OR2A4
Subfamily: Olfactory
Family: GPCR

Synonyms: OR2A4, Olfactory receptor 2A4, Olfactory receptor 2A10, Olfactory receptor OR6-37, OR2A10
Gen-ID 79541
Applikations-hinweise Approved: IHC, IHC-P (8 - 19 μg/mL)
Kommentare

Target Species of Antibody: Human

Testdurchführung

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Olfactory Receptor 2A4 (OR2A4) (Extracellular Domain) antibody (ABIN1049177) Anti-OR2A4 antibody ABIN1049177 IHC staining of human nasal mucosa, respiratory epit...
Immunohistochemistry (IHC) image for anti-Olfactory Receptor 2A4 (OR2A4) (Extracellular Domain) antibody (ABIN1049177) Anti-OR2A4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fix...
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