NPBWR1 Antikörper (Extracellular Domain)

Produktnummer ABIN1049110

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NPBWR1 in IHC (p), IHC und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für NPBWR1 Antikörper (Extracellular Domain) (ABIN1049110)

Target: NPBWR1 (Neuropeptides B/W Receptor 1 (NPBWR1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NPBWR1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-NPBWR1 Antikörper

Bindungsspezifität: Extracellular Domain

Marke: IHC-plus™

Spezifität: Human NPBWR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda (100%) Marmoset, Mouse, Rat, Bovine, Horse (95%) Rabbit (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from 2nd extracellular domain of human NPBWR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda (100%), Marmoset, Mouse, Rat, Bovine, Horse (95%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr, IHC-P (6 - 12 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-NPBWR1 Antikörper (ABIN1049110)

Singh, Maguire, Kuc, Fidock, Davenport: "Identification and cellular localisation of NPW1 (GPR7) receptors for the novel neuropeptide W-23 by [125I]-NPW radioligand binding and immunocytochemistry." in: Brain research, Vol. 1017, Issue 1-2, pp. 222-6, (2004) (PubMed).

Details zu NPBWR1

Target: NPBWR1 (Neuropeptides B/W Receptor 1 (NPBWR1))

Andere Bezeichnung: NPBWR1 / GPR7

Hintergrund: Name/Gene ID: NPBWR1
Subfamily: Neuropeptide
Family: GPCR

Synonyms: NPBWR1, G protein-coupled receptor 7, GPR7, Neuropeptide B/W receptor 1, Neuropeptides B/W receptor 1, G-protein coupled receptor 7

Gen-ID: 2831