MC1 Receptor Antikörper (Cytoplasmic Domain)
Kurzübersicht für MC1 Receptor Antikörper (Cytoplasmic Domain) (ABIN1049044)
Target
Alle MC1 Receptor (MC1R) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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                                            Bindungsspezifität
- Cytoplasmic Domain
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                                            Marke
- IHC-plus™
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                                            Spezifität
- Human MC1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
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                                            Homologie
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%) Baboon, Cat, Panda (94%).
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                                            Aufreinigung
- Immunoaffinity purified
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                                            Immunogen
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                        Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human MC1 Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Baboon, Cat, Panda (94%).
 Type of Immunogen: Synthetic peptide
 
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                                            Applikationshinweise
- Approved: IHC, IHC-P (2.5 - 5 μg/mL)
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                                            Kommentare
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                        Target Species of Antibody: Human 
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                                            Testdurchführung
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                        The IHC-pro Immunohistochemistry Protocol 
 Tissue Preparation
 Formalin fixation and embedding in paraffin wax
 Tissue Sectioning
 Make 4-µm sections and place on pre-cleaned and charged microscope slides.
 Heat in a tissue-drying oven for 45 minutes at 60°C
 Deparaffinization
 Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
 Rehydration
 Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
 Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
 Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
 Rinse slides in gentle running distilled water – 5 minutes at room temperature.
 Antigen retrieval
 Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
 Remove from heat and let stand at room temperature in buffer - 20 minutes
 Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
 Immunostaining
 Do not allow tissues to dry at any time during the staining procedure.
 Apply a universal protein block – 20 minutes at room temperature.
 Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
 Rinse slides in 1X TBST - 1 minute at room temperature.
 Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
 Rinse slides 1X TBST – 1 minute at room temperature.
 Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
 Rinse slides in 1X TBST - 1 minute at room temperature.
 Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
 Wash slides in distilled water – 1 minute at room temperature.
 Dehydrate
 This method should only be used if the chromogen substrate is alcohol insoluble.
 Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
 Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
 Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
 Wash slides in 3 changes of xylene – 1 minute each at room temperature.
 Apply coverslip
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                                            Beschränkungen
- Nur für Forschungszwecke einsetzbar
 
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                                            Format
- Liquid
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                                            Konzentration
- Lot specific
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                                            Buffer
- PBS, less than 0.1 % sodium azide.
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                                            Konservierungsmittel
- Sodium azide
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                                            Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            Lagerung
- 4 °C,-20 °C
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                                            Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
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                                            Haltbarkeit
- 12 months
 
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    - MC1 Receptor (MC1R) (Melanocortin 1 Receptor (MC1R))
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                                            Andere Bezeichnung
- MC1R
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                                            Hintergrund
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                        Name/Gene ID: MC1R
 Subfamily: Melanocortin
 Family: GPCR
 Synonyms: MC1R, CMM5, MC1 receptor, MC1-R, MSHR, Melanocortin receptor 1, Melanotropin receptor, Melanocortin 1 receptor, MSH-R, SHEP2
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                                            Gen-ID
- 4157
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                                            Pathways
- cAMP Metabolic Process, Feeding Behaviour
 Target
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