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MC1 Receptor Antikörper (Cytoplasmic Domain)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MC1 Receptor in IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Affe.
Produktnummer ABIN1049044

Kurzübersicht für MC1 Receptor Antikörper (Cytoplasmic Domain) (ABIN1049044)

Target

Alle MC1 Receptor (MC1R) Antikörper anzeigen
MC1 Receptor (MC1R) (Melanocortin 1 Receptor (MC1R))

Reaktivität

  • 65
  • 27
  • 11
  • 3
  • 2
  • 1
  • 1
Human, Affe

Wirt

  • 67
  • 10
Kaninchen

Klonalität

  • 72
  • 5
Polyklonal

Konjugat

  • 30
  • 8
  • 5
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser MC1 Receptor Antikörper ist unkonjugiert

Applikation

  • 54
  • 28
  • 26
  • 26
  • 14
  • 11
  • 9
  • 8
  • 7
  • 3
  • 2
  • 1
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 16
    • 15
    • 11
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain

    Marke

    IHC-plus™

    Spezifität

    Human MC1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

    Homologie

    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%) Baboon, Cat, Panda (94%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human MC1 Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Baboon, Cat, Panda (94%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise

    Approved: IHC, IHC-P (2.5 - 5 μg/mL)

    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    PBS, less than 0.1 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    MC1 Receptor (MC1R) (Melanocortin 1 Receptor (MC1R))

    Andere Bezeichnung

    MC1R

    Hintergrund

    Name/Gene ID: MC1R
    Subfamily: Melanocortin
    Family: GPCR

    Synonyms: MC1R, CMM5, MC1 receptor, MC1-R, MSHR, Melanocortin receptor 1, Melanotropin receptor, Melanocortin 1 receptor, MSH-R, SHEP2

    Gen-ID

    4157

    Pathways

    cAMP Metabolic Process, Feeding Behaviour
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