LTB4R2 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1049031

Dieses Anti-LTB4R2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LTB4R2 in FACS, IHC, IHC (p) und IHC (fro). Geeignet für Human und Affe. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für LTB4R2 Antikörper (Cytoplasmic Domain) (ABIN1049031)

Target: LTB4R2 (Leukotriene B4 Receptor 2 (LTB4R2))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LTB4R2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-LTB4R2 Antikörper

Bindungsspezifität: Cytoplasmic Domain

Marke: IHC-plus™

Spezifität: Human LTB4R2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Rat, Bat (88%) Elephant (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human LTB4R2. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Rat, Bat (88%), Elephant (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Flo, IHC, IHC-Fr, IHC-P (26 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-LTB4R2 Antikörper (ABIN1049031)

Bäck, Bu, Bränström, Sheikine, Yan, Hansson: "Leukotriene B4 signaling through NF-kappaB-dependent BLT1 receptors on vascular smooth muscle cells in atherosclerosis and intimal hyperplasia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 48, pp. 17501-6, (2005) (PubMed).

Details zu LTB4R2

Target: LTB4R2 (Leukotriene B4 Receptor 2 (LTB4R2))

Andere Bezeichnung: LTB4R2 / BLT2

Hintergrund: Name/Gene ID: LTB4R2
Subfamily: Chemoattractant
Family: GPCR

Synonyms: LTB4R2, BLT2, BLTR2, Blt-2, BLT2R, JULF2, LTB4-R2, Hblt2, KPG_004, Leukotriene B4 receptor 2, Leukotriene B4 receptor BLT2, LTB4 receptor JULF2, LTB4-R 2

Gen-ID: 56413

Pathways: cAMP Metabolic Process