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LTB4R2 Antikörper (N-Term)

Der Kaninchen Polyklonal Anti-LTB4R2-Antikörper wurde für IHC und IHC (p) validiert. Er ist geeignet, LTB4R2 in Proben von Human zu detektieren.
Produktnummer ABIN1049030

Kurzübersicht für LTB4R2 Antikörper (N-Term) (ABIN1049030)

Target

Alle LTB4R2 Antikörper anzeigen
LTB4R2 (Leukotriene B4 Receptor 2 (LTB4R2))

Reaktivität

  • 28
  • 27
  • 20
  • 5
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
Human

Wirt

  • 43
Kaninchen

Klonalität

  • 43
Polyklonal

Konjugat

  • 13
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser LTB4R2 Antikörper ist unkonjugiert

Applikation

  • 33
  • 18
  • 13
  • 13
  • 12
  • 5
  • 4
  • 2
  • 1
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 16
    • 8
    • 5
    • 2
    • 1
    • 1
    • 1
    N-Term

    Marke

    IHC-plus™

    Spezifität

    Human LTB4R2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PPM2C (47 % ).

    Homologie

    Percent identity with other species by BLAST analysis: Human, Gorilla (100%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic 19 amino acid peptide from N-terminal extracellular domain of human LTB4R2. Percent identity with other species by BLAST analysis: Human, Gorilla (100%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise

    Approved: IHC, IHC-P (10 - 20 μg/mL)

    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    PBS, less than 0.1 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    LTB4R2 (Leukotriene B4 Receptor 2 (LTB4R2))

    Andere Bezeichnung

    LTB4R2 / BLT2

    Hintergrund

    Name/Gene ID: LTB4R2
    Subfamily: Chemoattractant
    Family: GPCR

    Synonyms: LTB4R2, BLT2, BLTR2, Blt-2, BLT2R, JULF2, LTB4-R2, Hblt2, KPG_004, Leukotriene B4 receptor 2, Leukotriene B4 receptor BLT2, LTB4 receptor JULF2, LTB4-R 2

    Gen-ID

    56413

    Pathways

    cAMP Metabolic Process
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