GPER Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1048791

Produktdetails anti-GPER Antikörper

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPER Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Marke: IHC-plus™

Spezifität: Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Marmoset (94%) Monkey, Panda (88%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Marmoset (94%), Monkey, Panda (88%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (15 - 20 μg/mL), WB

Not recommended for: IF

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-GPER Antikörper (ABIN1048791)

Vivacqua, Bonofiglio, Recchia, Musti, Picard, Andò, Maggiolini: "The G protein-coupled receptor GPR30 mediates the proliferative effects induced by 17beta-estradiol and hydroxytamoxifen in endometrial cancer cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 20, Issue 3, pp. 631-46, (2006) (PubMed).

Thomas, Pang, Filardo, Dong: "Identity of an estrogen membrane receptor coupled to a G protein in human breast cancer cells." in: Endocrinology, Vol. 146, Issue 2, pp. 624-32, (2005) (PubMed).

Details zu GPER

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Andere Bezeichnung: GPER1 / GPR30 (GPER Produkte)

Hintergrund: Name/Gene ID: GPER1
Subfamily: Transmembrane Estrogen Receptor
Family: GPCR

Synonyms: GPER1, CMKRL2, CEPR, FEG-1, G-protein coupled receptor 30, G protein-coupled receptor 30, GPCR-Br, GPER, Heptahelix receptor, LERGU, LyGPR, MER, GPR30, IL8-related receptor DRY12, LERGU2, Chemokine receptor-like 2, DRY12, Membrane estrogen receptor

Gen-ID: 2852

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process