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GPER Antikörper (Cytoplasmic Domain)

Dieses Anti-GPER-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GPER in WB, IHC (p), IHC und ICC. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.
Produktnummer ABIN1048791

Kurzübersicht für GPER Antikörper (Cytoplasmic Domain) (ABIN1048791)

Target

Alle GPER Antikörper anzeigen
GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Reaktivität

  • 95
  • 63
  • 52
  • 5
  • 4
  • 3
  • 2
  • 1
Human

Wirt

  • 96
  • 1
  • 1
Kaninchen

Klonalität

  • 97
  • 1
Polyklonal

Konjugat

  • 36
  • 6
  • 6
  • 5
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
Dieser GPER Antikörper ist unkonjugiert

Applikation

  • 75
  • 43
  • 39
  • 39
  • 20
  • 15
  • 11
  • 9
  • 8
  • 8
  • 5
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 16
    • 16
    • 16
    • 9
    • 9
    • 7
    • 7
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain

    Marke

    IHC-plus™

    Spezifität

    Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

    Homologie

    Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Marmoset (94%) Monkey, Panda (88%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Marmoset (94%), Monkey, Panda (88%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise

    Approved: ICC, IHC, IHC-P (15 - 20 μg/mL), WB

    Not recommended for: IF

    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    PBS, less than 0.1 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Vivacqua, Bonofiglio, Recchia, Musti, Picard, Andò, Maggiolini: "The G protein-coupled receptor GPR30 mediates the proliferative effects induced by 17beta-estradiol and hydroxytamoxifen in endometrial cancer cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 20, Issue 3, pp. 631-46, (2006) (PubMed).

    Thomas, Pang, Filardo, Dong: "Identity of an estrogen membrane receptor coupled to a G protein in human breast cancer cells." in: Endocrinology, Vol. 146, Issue 2, pp. 624-32, (2005) (PubMed).

  • Target

    GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

    Andere Bezeichnung

    GPER1 / GPR30

    Hintergrund

    Name/Gene ID: GPER1
    Subfamily: Transmembrane Estrogen Receptor
    Family: GPCR

    Synonyms: GPER1, CMKRL2, CEPR, FEG-1, G-protein coupled receptor 30, G protein-coupled receptor 30, GPCR-Br, GPER, Heptahelix receptor, LERGU, LyGPR, MER, GPR30, IL8-related receptor DRY12, LERGU2, Chemokine receptor-like 2, DRY12, Membrane estrogen receptor

    Gen-ID

    2852

    Pathways

    EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
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