GPR137B Antikörper (Cytoplasmic Domain)
Kurzübersicht für GPR137B Antikörper (Cytoplasmic Domain) (ABIN1048718)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- Cytoplasmic Domain
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Marke
- IHC-plus™
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Spezifität
- Human GPR137B / TM7SF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
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Homologie
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard (100%) Goat, Panda, Xenopus, Zebrafish (94%) Platypus, Stickleback (88%) Pufferfish (81%).
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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Synthetic 16 amino acid peptide from 2nd cytoplasmic domain of human GPR137B / TM7SF1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard (100%), Goat, Panda, Xenopus, Zebrafish (94%), Platypus, Stickleback (88%), Pufferfish (81%).
Type of Immunogen: Synthetic peptide
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anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibody
GPR137B Reaktivität: Human, Maus, Ratte, Pferd, Affe, Schwein, Kaninchen IHC (p), IHC, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B Reaktivität: Human, Maus, Ratte, Pferd, Affe, Schwein, Kaninchen, Rind (Kuh), Hund, Hamster IHC (p), IHC Wirt: Kaninchen Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B Reaktivität: Human, Maus WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B Reaktivität: Human IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B Reaktivität: Human IHC (p), IF Wirt: Kaninchen Polyclonal unconjugated
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Applikationshinweise
- Approved: ICC (6 - 12 μg/mL), IHC, IHC-P (6 - 12 μg/mL)
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Kommentare
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Target Species of Antibody: Human
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Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- PBS, less than 0.1 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
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Haltbarkeit
- 12 months
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- GPR137B (G Protein-Coupled Receptor 137B (GPR137B))
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Andere Bezeichnung
- GPR137B
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Hintergrund
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Name/Gene ID: GPR137B
Subfamily: Orphan-U
Family: GPCR
Synonyms: GPR137B, TM7SF1 -
Gen-ID
- 7107
Target
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