NFYA Antikörper (N-Term)

Produktnummer ABIN104769

Hersteller Produkt- Nr.: 200-401-100

Dieses Anti-NFYA-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NFYA in WB und ELISA. Geeignet für Human und Affe. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für NFYA Antikörper (N-Term) (ABIN104769)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFYA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-NFYA Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 200-401-100

Hersteller: Rockland

Verwendungszweck: NF-Y Antibody

Kreuzreaktivität (Details): A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.

Produktmerkmale: Synonyms: rabbit anti-NF-Y Antibody, Nf-Y-A subunit, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A

Aufreinigung: Anti-NF-Y (a) antibody was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-NF-Y antibody is tested for western blotting and suitable for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-NFYA Antikörper (ABIN104769)

Falcone, Grandoni, Garibaldi, Manni, Filligoi, Piaggio, Gurtner: "Infinity: An In-Silico Tool for Genome-Wide Prediction of Specific DNA Matrices in miRNA Genomic Loci." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153658, (2016) (PubMed).

Gurtner, Fuschi, Magi, Colussi, Gaetano, Dobbelstein, Sacchi, Piaggio: "NF-Y dependent epigenetic modifications discriminate between proliferating and postmitotic tissue." in: PLoS ONE, Vol. 3, Issue 4, pp. e2047, (2008) (PubMed).

Cecchinelli, Lavra, Rinaldo, Iacovelli, Gurtner, Gasbarri, Ulivieri, Del Prete, Trovato, Piaggio, Bartolazzi, Soddu, Sciacchitano: "Repression of the antiapoptotic molecule galectin-3 by homeodomain-interacting protein kinase 2-activated p53 is required for p53-induced apoptosis." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4746-57, (2006) (PubMed).

Yu, Zhu, Pi, Ling, Ko, Takeda, Tuan: "The long terminal repeat (LTR) of ERV-9 human endogenous retrovirus binds to NF-Y in the assembly of an active LTR enhancer complex NF-Y/MZF1/GATA-2." in: The Journal of biological chemistry, Vol. 280, Issue 42, pp. 35184-94, (2005) (PubMed).

Details zu NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Andere Bezeichnung: NFYA

Hintergrund: Background: Anti-NF-Y antibody detects NF-Y (a subunit) protein. NF-Y is a highly conserved transcription factor that stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. NF-subunit A associates with a tight dimer composed of the NF-YB and NF-YC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. Anti-NF-YA Antibody is ideal for investigators involved in Cell Signaling, Immunology, Cancer, and Signal Transduction research.

Gen-ID: 4800

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha