Kaninchen anti-Hund IgG (Heavy & Light Chain) Antikörper (Texas Red (TR)) - Preadsorbed

Details zu Produkt Nr. ABIN1044015
Antigen
Epitop
Heavy & Light Chain
1570
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reaktivität
Hund
355
342
337
233
232
122
113
87
80
73
72
65
61
57
53
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Wirt
Kaninchen
846
798
336
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Klonalität
Polyklonal
Konjugat
Texas Red (TR)
236
220
209
150
147
129
75
49
48
35
31
26
26
26
26
26
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Optionen
Immunogen

Immunogen: Dog IgG whole molecule

Isotyp IgG
Fragment F(ab')2 fragment
Spezifität Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgG and Dog Serum.
Produktmerkmale This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reinigung Preadsorption: Solid phase absorption
Labeling Ratio 5.6
Antigen
Hintergrund

Synonyms: Rabbit F(ab')2 Anti-Dog IgG Antibody Texas Red™ Conjugation, Rabbit Fab2 Anti-Dog IgG Texas Red™ Conjugated Antibody

Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Applikations-hinweise

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:500-1:2,500

Kommentare

Post Translational Modification: Phosphorylation.
Texas Red™ is a registered trademark of Molecular Probes Inc.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Lagerung RT,4 °C,-20 °C
Informationen zur Lagerung Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for Rabbit anti-Dog (Canine) IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - Preadsorbed (ABIN1044015) Western Blot of Rabbit anti-Dog IgG Texas Red Conjugated Antibody. Lane 1: Dog IgG. L...