Kaninchen anti-Ziege IgG (Heavy & Light Chain) Antikörper (Atto 488) - Preadsorbed

Details zu Produkt Nr. ABIN1043941
Antigen
Epitop
Heavy & Light Chain
1570
134
100
57
34
33
25
6
6
6
6
5
4
4
4
3
3
1
1
1
Reaktivität
Ziege
355
342
337
233
232
122
113
87
80
73
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Wirt
Kaninchen
846
798
337
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Klonalität
Polyklonal
Konjugat
Atto 488
236
220
209
150
147
130
75
50
48
35
31
26
26
26
26
26
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Optionen
Immunogen

Immunogen: Goat IgG whole molecule

Isotyp IgG
Spezifität Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
Produktmerkmale This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reinigung Preadsorption: Solid phase absorption
Labeling Ratio 3.8
Antigen
Hintergrund

Synonyms: rabbit anti-Goat IgG ATTO 488 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO488 Conjugation

Background: Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Applikations-hinweise

Application Note: GOAT IgG (H&L) conjugated to ATTO 488 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Kommentare

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Lagerung RT,4 °C,-20 °C
Informationen zur Lagerung Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed (ABIN1043941) Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed (Image 1)
Image no. 2 for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed (ABIN1043941) Western Blot of Rabbit anti-Goat IgG Atto488 Conjugated Antibody. Lane 1: Goat IgG. L...