IL-33 Antikörper (Biotin)

Produktnummer ABIN1043805

Hersteller Produkt- Nr.: 209-406-b50

Dieses Anti-IL-33-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IL-33 in WB, ELISA und IHC. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für IL-33 Antikörper (Biotin) (ABIN1043805)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-33 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-IL-33 Antikörper (Biotin)

Hersteller Produkt- Nr.: 209-406-b50

Hersteller: Rockland

Verwendungszweck: IL-33 Antibody Biotin Conjugated

Kreuzreaktivität (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids.

Produktmerkmale: Synonyms: rabbit anti-Interleukin-33 biotin conjugated antibody, rabbit anti-IL-33 biotin conjugated antibody, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV

Aufreinigung: This purified antibody has been heated to 56°C for 30 minutes.

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 18 kDa in size corresponding to mature human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-IL-33 Antikörper (Biotin) (ABIN1043805)

Hayakawa, Hayakawa, Matsuyama, Tamemoto, Okazaki, Tominaga: "Mature interleukin-33 is produced by calpain-mediated cleavage in vivo." in: Biochemical and biophysical research communications, Vol. 387, Issue 1, pp. 218-22, (2009) (PubMed).

Details zu IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Andere Bezeichnung: IL33

Hintergrund: Background: IL-33 (also known as Interleukin-33, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV) is a cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces T helper type 2-associated cytokines. IL-33 is a secreted cytokine that is expressed at high levels in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes and is almost undetectable in placenta. The 31 kDa precursor is proteolytically converted to an 18 kDa mature form by CASP1.

Gen-ID: 90865, 15559209

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response