CCL4 Antikörper (HRP)

Produktnummer ABIN1043803

Produktdetails anti-CCL4 Antikörper (HRP)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL4 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Spezifität: Human MIP-1 beta Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-9 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Produktmerkmale: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated. Immunology Research

Aufreinigung: purified

Immunogen: MIP-1 beta Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1ß protein.
Immunogen Type: RecombinantProtein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to human MIP-1ß protein by western blotting in the appropriate cell lysate or extract. Immunology Research

Kommentare:

Gene Name: CCL4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Handhabung: Do NOT add Sodium Azide!

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-CCL4 Antikörper (HRP) (ABIN1043803)

Guan, Wang, Roderiquez, Norcross: "Natural truncation of the chemokine MIP-1 beta /CCL4 affects receptor specificity but not anti-HIV-1 activity." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 32348-52, (2002) (PubMed).

Details zu CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Andere Bezeichnung: MIP-1 beta (CCL4 Produkte)

Synonyme: ACT2 antikoerper, AT744.1 antikoerper, G-26 antikoerper, HC21 antikoerper, LAG-1 antikoerper, LAG1 antikoerper, MIP-1-beta antikoerper, MIP1B antikoerper, MIP1B1 antikoerper, SCYA2 antikoerper, SCYA4 antikoerper, CCL4 antikoerper, K203 antikoerper, Mip1-b antikoerper, Scya4 antikoerper, MIP-1beta antikoerper, MIP-1BETA antikoerper, MIP1b antikoerper, SCY4A antikoerper, Act-2 antikoerper, MIP-1B antikoerper, Mip1b antikoerper, ACT-2 antikoerper, AT744.2 antikoerper, CCL4L antikoerper, SCYA4L antikoerper, SCYA4L1 antikoerper, SCYA4L2 antikoerper, C-C motif chemokine ligand 4 antikoerper, chemokine (C-C motif) ligand 4 antikoerper, chemokine (C-C motif) ligand 4-like 1 antikoerper, C-C motif chemokine ligand 4 like 1 antikoerper, CCL4 antikoerper, Ccl4 antikoerper, CCL4L1 antikoerper

Hintergrund: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated. Immunology Research
Synonyms: CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, T-cell activation protein 2, Protein H400, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, MIP-1ß

Gen-ID: 6351

NCBI Accession: NP_002975

UniProt: P13236