Interleukin 17a Antikörper (Biotin)

Produktnummer ABIN1043792

Hersteller Produkt- Nr.: 209-306-b32

Der Maus Monoklonal Anti-Interleukin 17a-Antikörper wurde für ELISA, WB und FACS validiert. Er ist geeignet, Interleukin 17a in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Interleukin 17a Antikörper (Biotin) (ABIN1043792)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interleukin 17a Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Klon: 4H1524-1

Produktdetails anti-Interleukin 17a Antikörper (Biotin)

Hersteller Produkt- Nr.: 209-306-b32

Hersteller: Rockland

Verwendungszweck: IL-17A Biotin Conjugated Antibody

Kreuzreaktivität (Details): This antibody is specific for human IL-17A protein.

Produktmerkmale: Synonyms: mouse anti-Interleukin-17A biotin conjugated antibody, mouse anti-IL-17A biotin conjugated antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17

Aufreinigung: This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-IL-17A (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development.

Immunogen Type: Recombinant Protein

Isotyp: IgG2b

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Application Note: This purified antibody has been tested by Western Blot and suitable for use in FLOW. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Interleukin 17a Antikörper (Biotin) (ABIN1043792)

Paust, Turner, Steinmetz, Peters, Heymann, Hölscher, Wolf, Kurts, Mittrücker, Stahl, Panzer: "The IL-23/Th17 axis contributes to renal injury in experimental glomerulonephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 20, Issue 5, pp. 969-79, (2009) (PubMed).

Details zu Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Andere Bezeichnung: IL17A

Hintergrund: Background: IL17-A (also known as Interleukin-17) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

Gen-ID: 3605

NCBI Accession: NP_002181

UniProt: Q16552