TIMP4 Antikörper
Quick Overview for TIMP4 Antikörper (ABIN1043743)
Target
Alle TIMP4 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Hersteller Produkt- Nr.
- 200-301-e65
-
Hersteller
- Rockland
-
Verwendungszweck
- TIMP4 Antibody
-
Kreuzreaktivität (Details)
- TIMP4 antibody is specific for human TIMP4 protein.
-
Produktmerkmale
- Synonyms: mouse anti-TIMP4 antibody, Tissue inhibitor of metalloproteinase-4, Metalloproteinase inhibitor 4, TIMP-4, TIMP 4, TIMP4 antibody, anti-TIMP-4 antibody
-
Aufreinigung
- Anti-TIMP4 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above.
-
Sterilität
- Sterile filtered
-
Immunogen
-
Immunogen: Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full length human TIMP4 protein followed by hybridoma development.
Immunogen Type: Recombinant Protein
-
Isotyp
- IgG2b kappa
-
-
-
-
Applikationshinweise
-
Immunohistochemistry Dilution: 5-10 μg/mL
Application Note: Anti-TIMP4 antibody has been tested for use in ELISA, Native - Western, and IHC (P). SDS-PAGE - Western Blot not useful for endogenous detection. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:2,000 to 1:10,000
ELISA Dilution: 1:20,000 to 1:100,000
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konzentration
- 1.0 mg/mL
-
Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Lagerung
- 4 °C,-20 °C
-
Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
Haltbarkeit
- 12 months
-
-
-
Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues." in: Journal of cellular biochemistry, Vol. 110, Issue 5, pp. 1255-61, (2010) (PubMed).
: "
-
Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues." in: Journal of cellular biochemistry, Vol. 110, Issue 5, pp. 1255-61, (2010) (PubMed).
-
- TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))
-
Andere Bezeichnung
- TIMP4
-
Hintergrund
- Background: Tissue inhibitor of metalloproteinase-4 (TIMP-4) is member of the TIMP family of inhibitors of matrix metalloproteinase (MMP) activities. In addition, TIMPs have non-MMP associated functions, including roles in growth promotion, apoptosis, and angiogenesis. All four members of the TIMP gene family form a similar secondary structure due to the formation of six highly conserved disulfide bonds. It has been reported that some TIMPs, TIMP4 included, can bind a tetraspanin in the plasma membrane, initiating an intra-cellular signaling cascade. Elevated levels of TIMP4 have been associated with breast cancer progression and heart disease.
-
Gen-ID
- 7079
-
NCBI Accession
- NM_003256
-
UniProt
- Q99727
Target
-