TIMP4 Antikörper

Produktnummer ABIN1043743

Hersteller Produkt- Nr.: 200-301-e65

Produktdetails anti-TIMP4 Antikörper

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TIMP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 200-301-e65

Hersteller: Rockland

Verwendungszweck: TIMP4 Antibody

Kreuzreaktivität (Details): TIMP4 antibody is specific for human TIMP4 protein.

Produktmerkmale: Synonyms: mouse anti-TIMP4 antibody, Tissue inhibitor of metalloproteinase-4, Metalloproteinase inhibitor 4, TIMP-4, TIMP 4, TIMP4 antibody, anti-TIMP-4 antibody

Aufreinigung: Anti-TIMP4 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full length human TIMP4 protein followed by hybridoma development.

Immunogen Type: Recombinant Protein

Klon: 9:4-7

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 5-10 μg/mL

Application Note: Anti-TIMP4 antibody has been tested for use in ELISA, Native - Western, and IHC (P). SDS-PAGE - Western Blot not useful for endogenous detection. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2,000 to 1:10,000

ELISA Dilution: 1:20,000 to 1:100,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-TIMP4 Antikörper (ABIN1043743)

Donover, Wojciechowski, Thirumaran, Zemba-Palko, Prendergast, Wallon: "Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues." in: Journal of cellular biochemistry, Vol. 110, Issue 5, pp. 1255-61, (2010) (PubMed).

Details zu TIMP4

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Andere Bezeichnung: TIMP4 (TIMP4 Produkte)

Hintergrund: Background: Tissue inhibitor of metalloproteinase-4 (TIMP-4) is member of the TIMP family of inhibitors of matrix metalloproteinase (MMP) activities. In addition, TIMPs have non-MMP associated functions, including roles in growth promotion, apoptosis, and angiogenesis. All four members of the TIMP gene family form a similar secondary structure due to the formation of six highly conserved disulfide bonds. It has been reported that some TIMPs, TIMP4 included, can bind a tetraspanin in the plasma membrane, initiating an intra-cellular signaling cascade. Elevated levels of TIMP4 have been associated with breast cancer progression and heart disease.

Gen-ID: 7079

NCBI Accession: NM_003256

UniProt: Q99727