BIN1 Antikörper

Produktnummer ABIN1043739

Hersteller Produkt- Nr.: 200-301-e63

Dieses Anti-BIN1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von BIN1 in WB, IHC, ELISA und IP. Geeignet für Maus. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für BIN1 Antikörper (ABIN1043739)

Target: BIN1 (Bridging Integrator 1 (BIN1))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BIN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Klon: 2F11

Produktdetails anti-BIN1 Antikörper

Hersteller Produkt- Nr.: 200-301-e63

Hersteller: Rockland

Verwendungszweck: BIN1 Antibody

Kreuzreaktivität (Details): BIN1 antibody is specific for human BIN1 protein.

Produktmerkmale: Synonyms: mouse anti-BIN1 Antibody, AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN 1, BIN-1, BIN1 antibody, anti-BIN1 antibody

Aufreinigung: Anti-BIN1 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with chimeric protein that encoded the human BIN1 BAR domain followed by hybridoma development.

Immunogen Type: Recombinant Protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-BIN1 antibody has been tested for use in Western Blot, ELISA, IP, and IHC. This antibody is suitable for. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:1500

Immunoprecipitation Dilution: 10-100 μL

ELISA Dilution: 1:5000-1:50000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-BIN1 Antikörper (ABIN1043739)

An, Gilani, Huang, Muncan, Zhang, Tang, Gerdes, Ojamaa: "Adverse transverse-tubule remodeling in a rat model of heart failure is attenuated with low-dose triiodothyronine treatment." in: Molecular medicine (Cambridge, Mass.), Vol. 25, Issue 1, pp. 53, (2020) (PubMed).

Nicot, Toussaint, Tosch, Kretz, Wallgren-Pettersson, Iwarsson, Kingston, Garnier, Biancalana, Oldfors, Mandel, Laporte: "Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy." in: Nature genetics, Vol. 39, Issue 9, pp. 1134-9, (2007) (PubMed).

Details zu BIN1

Target: BIN1 (Bridging Integrator 1 (BIN1))

Andere Bezeichnung: BIN1

Hintergrund: Background: Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. ) Bin1 may act with cancer suppressor and inhibits malignant cell transformation. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Bin1 has also been implicated in Alzheimer disease and cardiac disease. Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive, also known as autosomal recessive myotubular myopathy.

Gen-ID: 274

NCBI Accession: NP_004296

UniProt: O00499