gamma-Tubulin GCP2 (AA 2-194) Antikörper

Produktnummer ABIN1043030

Der Maus Monoklonal Anti--Antikörper wurde für ICC, IHC (p), IP und WB validiert. Er ist geeignet, in Proben von Human, Maus, Schwein und Ratte zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für gamma-Tubulin GCP2 (AA 2-194) Antikörper (ABIN1043030)

Target: gamma-Tubulin GCP2

Reaktivität: Human, Maus, Schwein, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Klon: GCP2-01

Produktdetails

Bindungsspezifität: AA 2-194

Verwendungszweck: Anti-gamma-Tubulin GCP2 Purified

Spezifität: The mouse monoclonal antibody GCP2-01 recognizes gamma-tubulin complex component 2 (GCP2), a 95-103 kDa intracellular protein. It recognizes also phosphorylated GCP2.

Kreuzreaktivität (Details): Human, Porcine, Mouse, Rat

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: GST-fusion protein containing amino acids 2-194 of mouse GCP2

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen

Dráberová, DAgostino, Caracciolo, Sládková, Sulimenko, Sulimenko, Sobol, Maounis, Tzelepis, Mahera, K?en, Legido, Giordano, Mörk, Hozák, Dráber, Katsetos: "Overexpression and Nucleolar Localization of ?-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma." in: Journal of neuropathology and experimental neurology, Vol. 74, Issue 7, pp. 723-42, (2015) (PubMed).

Ho?ejší, Vinopal, Sládková, Dráberová, Sulimenko, Sulimenko, Vosecká, Philimonenko, Hozák, Katsetos, Dráber: "Nuclear ?-tubulin associates with nucleoli and interacts with tumor suppressor protein C53." in: Journal of cellular physiology, Vol. 227, Issue 1, pp. 367-82, (2011) (PubMed).

Antigendetails

Target: gamma-Tubulin GCP2

Hintergrund: Tubulin gamma complex associated protein 2,Gamma tubulin complex component 2 (GCP2) is 95-103 kDa protein which associates with two molecules of gamma tubulin and one molecule of gamma tubulin complex component 3 (GCP3), to form the gamma tubulin small complexes (gammaTuSCs). These complexes interact with GCP4, 5, and 6 to form the gamma tubulin ring complexes (gammaTuRCs), which are embedded in the microtubule organizing center matrix where they nucleate microtubules. The gammaTuRCs are also involved in the regulation of microtubule plus and minus end dynamics. Components of gamma tubulin complexes are highly conservative in eukaryotes.,GCP-2, GTCC2, GTC2

Gen-ID: 10844

UniProt: Q9BSJ2